Clearly, the progenitors of ICC were located inside of the AP (Fig. S2). However, in the narrow segment of the HSCR colons, it was very difficult to detect c-Kit positive mature ICC and the c-Kit+/CD34+/Igf1r+ cells could not be located by overlapping fluorescence images in AP (Fig. 1I–L). This evidence concerns the gene IGF1R and intrahepatic cholangiocarcinoma.