Therefore, in the present study, the modified SB-based gene delivery system was used to transfer the suicide gene (HSV-TK) into multiple types of cancer cells, including H358 (lung cancer), H1299 (lung cancer), PC3 (prostate cancer), DU145 (prostate cancer), and OVCAR3 (ovarian cancer) cells with various experimental approaches including TUNEL assay, cell viability assay, FACS analysis, and in vivo experiment. This evidence concerns the gene TKT and cancer.