The reporter assays, ChIP experiments, and LMO2 knockdowns suggested that HHEX was a direct transcriptional target of LMO2. To analyze the role of Hhex in the development of Lmo2-induced T-ALL, we bred the CD2-Lmo2 transgenic mice to floxed Hhex (Hhexlox/lox) or to Hhexlox/lox; Vav-iCre (conditional knockouts termed Hhex cKO, Figure S6 in File S1). This evidence concerns the gene LMO2 and acute lymphoblastic leukemia.