Using FACs analysis of freshly isolated, non-permeabilized anchorage-independent CSCs to ascertain DEspR-expression on the cell membrane of Panc1-CSCs and U87-CSCs respectively (Figure 4A), we detected co-expression of DEspR and CD133, a known PDAC and GBM CSC-marker [35]. This evidence concerns the gene PROM1 and glioblastoma.