In Figure 1a we show that, even though a single iDC injection can transiently reverse new-onset T1D (return to diabetes as early as one week after insulin withdrawal), multiple injections are required for stable, long-term restoration of blood glucose to levels that reflect pre-diabetic conditions (non-fasting glucose levels were, on average, 218+/−63 mg/dL when combining all time points measured; n = 9 NOD mice at 22 weeks). Here, INS is linked to diabetes mellitus.