As shown in Figures 5F and G and 6F and G, despite lower expression of TLR2 and TLR4 in the sham group, TLR2 and TLR4 were observed to be located in neurons, as well as in astroglia and microglia in the ischemia group, indicating that neurons, astroglia and microglia participate in the inflammatory response caused by ischemia and reperfusion. This evidence concerns the gene TLR4 and ischemia.