We compared the SPTAN1 expression level of these cell lines with their corresponding MLH1 deficient cell lines, HEK293T [32] or the stably pcDNA3.1+ mock control transfected HCT116 mlh0-1 cell line [31], respectively and with the MLH1 deficient colorectal cancer cell line RKO using Western blot analysis. The gene discussed is SPTAN1; the disease is colorectal cancer.