Since we previously identified protein-protein interaction of MLH1 and SPTAN1 [12] and verified this interaction using HEK293 cells (Additional file 1), we analyzed the influence of MLH1 on the protein level of interacting SPTAN1 by using nine different human cancer cell lines: six MLH1 proficient and three MLH1 deficient ones. This evidence concerns the gene SPTAN1 and cancer.