The major findings of this study are as follows: (1) NAC treatment attenuates hypertension development in young SHR; (2) NAC administration results in a reduction in plasma ADMA levels, a decrease in superoxide production, and an increase in DDAH activity and the GSH/GSSG ratio in the kidneys of SHR; and (3) NAC and GSH both increase renal DDAH activity in vitro. Here, DDAH2 is linked to Hypertension.