Figure 4B confirms the above observations and shows the increase in MFI in lepromatous as compared to tuberculoid subjects for both FOXP3 (p<0.002) and TGF-β (p<0.01) in the CD4+CD25+ T cells in antigen stimulated PBMC cultures. That the iTreg discrimination noted in the two leprosy types was driven by M.leprae antigens was indicated by PHA stimulated PBMC cultures which showed general increase but not statistically significant differences between the two leprosy types either in percentage of cells with lineage specific markers or in MFI for FOXP3 and TGF-β (Figure S3). This evidence concerns the gene TGFB1 and leprosy.