MGMT and neoplasm: The provocative clinical relevance of MGMT promoter status has led to an ongoing debate over the establishment of a standardized test method, which is suitable for high-throughput analysis from small amounts of DNA samples (e.g., formalin-fixed, paraffin-embedded tumor tissues) and highly reproducible in independent laboratories.[37] Two assay methods, i.e., gel-based and real-time MSP, were used in the included studies.