However, this seemed unlikely, given that Fpn1Alb/Alb mice (which were first pre-loaded with iron, and then transferred to an iron-poor diet to drive tissue iron mobilization) had liver iron loading and decreased Bmp6 and Hamp1 expression, yet developed no significant signs of anemia (Table S4 in File S1) or changes in Epo expression compared to control Fpn1flox/flox mice (Figure 4E). Here, EPO is linked to anemia.