In consistent with the results of Cav-1 overexpression experiments, knockdown of Cav-1 repressed Twist expression (Fig. 3A and B) and didn't influence the expression of both Slug and SNAI1 in SNU449 cells (Figure S1A), which verified further that Twist took part in Cav-1-driven EMT of HCC cells. This evidence concerns the gene TWIST1 and hepatocellular carcinoma.