To determine whether Cav-1 plays an important role in GLI1-driven EMT of HCC cells, we inhibited Cav-1 up-regulation induced by forced expression of GLI1 in Huh7 cells through transfecting Cav-1 siRNAs (Fig. 4C) and found it leaded to up-regulation of E-cadherin and down-regulation of N-cadherin, Fibronectin and Vimentin in Huh7 GLI1 cells (Fig. 4C), which states clearly that inhibition of Cav-1 reverses EMT phenotype of HCC cells induced by GLI1. This evidence concerns the gene FN1 and hepatocellular carcinoma.