CFL1 and melanoma: Figure 1A and Movie S1 show a B16 melanoma cell expressing GFP-actin that was infected with baculovirus tagged with mCherry [14]. The actin comet tails formed were indistinguishable from those seen in the native host cells, moved with a velocity of up to 50 μm/min, and contained typical tail components (Figures 1B and S1) [11]. From intensity measurements of tails in cells transfected with different combinations of GFP/mCherry-tagged proteins, VASP and ArpC5 co-localized with actin and capping protein β and cofilin trailed behind the actin label (Figure 1B and Figure S1).