In univariate analyses of tumor tissue OPN, when defined and assessed as the percentage of cells staining by IHC, there was no association between primary tumor OPN expression with cut-point at mean and EFS (P = 0.34), RFS (P = 0.88), OS (P = 0.62), bone RFS (P = 0.74) or non-bone RFS (P = 0.96), nor was inflammatory cell OPN associated with any endpoint (respectively, P = 0.55, 0.26, 0.10, 0.27, 0.75). This evidence concerns the gene SPP1 and neoplasm.