Another study showed that MDA-MB-231 cells grown as spheroids, do not exhibit the adhesive phenotype seen by the less invasive breast cancer cells MCF7 and MCF10A; however, adhesion of MDA-MB-231 spheroid-grown cells to E-Selectin can be induced by treatment with plasma, interleukin 6, or tumor necrosis factor alpha 86. This evidence concerns the gene TNF and breast cancer.