To further determine whether miR-30a directly targets vimentin mRNA in gastric cancer cells, we inserted the full-length 3′ UTR of vimentin into the pMIR-REPORT luciferase vector (pMIR-Vim; Fig. 6C) and investigated the effect of miR-30a on the luciferase activity of pMIR-Vim. This evidence concerns the gene VIM and gastric cancer.