Adipose tissue expression of the marker genes specific for pan-T cells (Cd3e), cytotoxic (Cd8a), and regulatory T cells (Foxp3) as well as B cells (Cd19) revealed no significant obesity-induced alterations after both high-fat diets compared to lean LF-fed LDLR-/- mice (Additional file 4: Figure S2). The gene discussed is LDLR; the disease is obesity due to melanocortin 4 receptor deficiency.