In one study, radiation of subcutaneously inoculated B16-OVA at tumor sites led to increased priming of tumor-reactive T cells in the draining lymph node and accumulation of endogenous tumor antigen-specific CD8+ and CD4+ T cells infiltrating the tumor; adoptive transfer of ex vivo activated OVA-specific OT-1 CD8+ T cells led to increased infiltration of transferred T cells to tumor sites (and not merely expansion of localized T cells) following local radiation and was particularly evident with single dose (15 Gy), compared with fractionated dose deliver [41]. This evidence concerns the gene CD8A and neoplasm.