Quantitative RT-PCR was used to measure the expression of 9 genes deregulated on both the 15 k custom and the 44 k commercial microarrays, and the differential expression of all genes in SRSF2-over-expressing H358 lung cancer cells in comparison to H358 control cells was analyzed with RNA isolated independently from that used for chip hybridization (Additional file7: Table S6). The gene discussed is SRSF2; the disease is lung cancer.