In addition, the ratio of nNOS-expressing neurons to total HuC/D immunoreactive neurons was significantly decreased in the myenteric ganglia from NEC patients compared with bowel atresia patients both during the acute episode (0.26 ± 0.04 vs. 0.34 ± 0.03) and later during stoma closure (0.29 ± 0.03 vs. 0.36 ± 0.02) (Figure 2D). This evidence concerns the gene NOS1 and necrotizing enterocolitis.