SERPINB1 and otitis media: To delineate the role of pilus-1 in otitis media we used the chinchilla of experimental otitis media model (EOM) and evaluated colonization and disease due to the wild type Streptococcus pneumoniae strain Taiwan19F-14 wt and its otherwise isogenic Taiwan19F-14 ΔPI-1/PI-2 deficient mutant as well as the potential for RrgB321 to prevent middle ear (ME) disease.