Moreover, endogenous upregulation of Sirt1was observed mostly in retinal ganglion cells[12], the neuronal cell type most closely associated with superficial layer of retinal vessels impacted in retinopathy, while as over-expression of Nestin-driven Sirt1was found in pan-retinal neurons, most significantly observed in inner nuclear layer composed mainly of bipolar cells. The gene discussed is NES; the disease is retinal disorder.