Of the new ones, five were in TMC1, two in USH1C, two in CDH23 and four in TMIE. The variants classified as benign satisfied one or more of the following criteria: (i) allele frequency of 0.01 or more among the control individuals; (ii) lack of segregation with the deafness phenotype; (iii) poor evolutionary conservation and (iv) apparently no effect on transcript or protein function (Table S1). This evidence concerns the gene TMC1 and deafness.