AQP4 and Cerebral ischemia: In addition, on the basis of the Western blotting results, it can be estimated that mislocalization of AQP4 anchoring at the perivascular membrane after cerebral ischemia rather than a net loss of AQP4, results in a disruption of AQP4, which was quantified using immuno-electron microscopy and the minor difference in Western blotting analyses.