Interestingly, on the basis of the Western blot result at 3 days after ischemia, the expression of AQP4 in the TT/Stroke group was nearly similar to the Stroke group., The long-term effects of AQP4 expression after cerebral ischemia regulated by exercise preconditioning remains unclear and should be studied further. The gene discussed is AQP4; the disease is Cerebral ischemia.