As predicted by several in silico methods for target gene prediction, including PicTar (http://www.pictar.org/), TargetScan (http://www.targetscan.org), and microRNA (http://www.microrna.org/), the proliferation-associated oncogene implicated in malignant liver cancer growth induced by HCV infection, Wnt1, was identified as one of the high-scoring candidate genes of miR-152 targets, and as was shown in Figure 3A, two miR-152 binding sites (respectively located at 262–268 bp and 688–694 bp 3′-UTR) were indicated at 3′-UTR of the Wnt1 mRNA. The gene discussed is WNT1; the disease is liver cancer.