As can be seen in Figure 2B, there was a significant loss of CDC25A in MVM infected A9 cells beginning at 19 h pi, when virus replication (as evidenced by NS1 expression) became prominent, and continued through 25 hours post infection (h pi) (Figure 2B, panels a and d, compare lanes 2 and 4 with lanes 1 and 3). Here, CDC25A is linked to infection.