We depleted endogenous CPSF6 and infected the cells either with CA mutants that become dependent on cell division (E45A, Q63A/Q67A, A92E, G94D, and Q219A) or those that are impaired for infection but maintain cell cycle independence for infection (P38A, E71A, E128A/R132A and R143A) [38]. The gene discussed is CPSF6; the disease is infection.