To study the therapeutic potential of targeting DUSP1 in fully established pancreatic tumors, we stably transduced COLO-357 human pancreatic cancer cells with lentivirus expressing doxycycline-inducible shRNA against DUSP1 or a non-targeting scramble control, before injecting the cells into the pancreas of immunodeficient mice in a TET-OFF state. Here, DUSP1 is linked to pancreatic neoplasm.