To investigate that the adoptive transfer of IL-27R-deficient γδ T cells that are nonresponsive to IL-27 should improve anti-pneumococcal defence in WT mice with postinfluenza pneumococcal infection, γδ T cells were purified from IL-27R-deficient or WT mice by cell sorting (supplementary Fig 7), and were transferred intratracheally (i.t.)into WT mice following primary influenza infection, and then mice were intranasally challenged with S. pneumoniae (Fig 4F). This evidence concerns the gene IL27 and pneumococcal infection.