Low levels of FOXP3 mRNA (2-5 copies per 10,000 copies of beta-actin) were detected in 16 of the 25 melanoma cell lines, representing expression 300- to 1000- fold lower than the level observed in Treg cells (2000 copies per 10,000 copies of beta-actin) (Figure 1D). This evidence concerns the gene FOXP3 and melanoma.