Human MMR-deficient and -proficient CRC cells (HCT116, HCT116+ch3) and their respective 5-FU chemo-resistant derivative cell lines (HCT116R, HCT116+ch3R) were used in our study to investigate the effect of curcumin or/and 5-FU on cell viability, apoptosis and cancer stem cells (CSC) in high density cultures. Here, MRC1 is linked to colorectal carcinoma.