We analyzed RAB25 mRNA levels in six HNSCC cell lines by quantitative RT-PCR (UM-SCC-22A, UM-SCC-22B, UM-SCC-74B, FaDu, SCC9, and SCC25) and in OKF6-Tert1 immortalized normal keratinocytes. The gene discussed is RAB25; the disease is head and neck squamous cell carcinoma.