Regarding other preimmunization strategies, Filatenkov et al.26 vaccinated the donors with a subcutaneous injection of irradiated tumor cells and CpG-enriched oligonucleotides, which are adjuvants that stimulate DCs via TLR-9, and Brody et al.7 described in situ vaccination combining chemotherapy with intratumoral injection of CpG in murine models. Here, TLR9 is linked to neoplasm.