At the same time, we also used the CRC tissue array [24] to detect the expression of miR-429 by ISH and Onecut2 by IHC staining, and the results showed that miR-429 existed in cytoplasm and its expression was significantly decreased in CRC relative to paracancerous normal tissues; Onecut2 existed in nucleus and its expression was significantly increased in CRC relative to paracancerous normal tissues (Fig. 2e). Here, ONECUT2 is linked to colorectal carcinoma.