We found that the level of IL-1β mRNA was not elevated in HCV (JFH-1) infected Huh7 cells (Figure 1A), nor was the IL-1β protein being detected in SNs from these cells at day 1, day 2 or day 4 after virus infection (Figure 1B), although the infection efficiency was found normal as indicated by HCV RNA replication (Figure 1C). This evidence concerns the gene IL1B and infection.