Compared with the corresponding noncancerous bone tissues, the expression level of miR-340 (mean ± S.D.: 2.69 ± 1.01 vs. 4.11 ± 1.36, p < 0.001, Figure 3C) was significantly decreased, while the expression level of ROCK1 protein (mean ± S.D.: 1.40 ± 0.83 vs. 3.74 ± 1.25, p < 0.001, Figure 3D) was significantly increased in osteosarcoma tissues, which was similar with the results of qRT-PCR. This evidence concerns the gene ROCK1 and osteosarcoma.