Compared with noncancerous bone tissues, miR-340 expression was significantly decreased in osteosarcoma tissues (mean ± SD, osteosarcoma vs. noncancerous bone: 2.88 ± 1.11 vs. 4.46 ± 0.94, p < 0.001, Figure 1A), while ROCK1 expression was significantly increased in osteosarcoma tissues (mean ± SD, osteosarcoma vs. noncancerous bone: 5.11 ± 0.58 vs. 2.49 ± 1.11, p < 0.001, Figure 1B). Here, ROCK1 is linked to osteosarcoma.