In addition, the median values of miR-340 (2.88) and ROCK1 mRNA (5.10) expression levels in all osteosarcoma tissues were used as cutoff points to classify 92 patients with osteosarcomas into miR-340-low (n = 50), miR-340-high (n = 42), ROCK1-low (n = 40) and ROCK1-high (n = 52) expression groups. The gene discussed is ROCK1; the disease is osteosarcoma.