In addition, in the comparison between the Ad-CAG-REIC and Ad-SGE-REIC vectors by a western blot analysis in multiple human cancer cell lines, robust upregulation of REIC/Dkk-3 expression was observed in the Ad-SGE-REIC-transfected cells, particularly after the transfection at 10 MOI (Fig. 2B). Here, DKK3 is linked to cancer.