DAXX and in situ carcinoma: In normal cervical epithelial cells, Daxx distributed in the nucleus, and only located in the nucleus (Figure 1A); in HR-HPV positive cells of CIN, Daxx mainly distributed nearby the nuclear membrane, a little within the nucleus (Figure 1B); in HR-HPV positive cells of CIN, CIN, carcinoma in situ and invasive cervical cancer, Daxx densely distributed in the cytoplasm and cell membrane (Figure 1C, D, E, F, G, H).