Immunofluorescent staining to caspase-3 and DAPI disclosed presence of caspase-3- positive cells in dilated tubules and cysts of proximal and distal tubules, and in the interstitium of both CNF (Figure 2G-I) and FSGS kidneys (Figure 3F-H). This evidence concerns the gene CASP3 and focal segmental glomerulosclerosis.