TRAP enzyme activity in osteocytes (ELF97+ Ot/Ot) was increased in cancellous versus cortical bone in all groups from both animal models, p < 0.01 (Ovx-D, sham, rickets, healing after 48 h, and controls), p < 0.05 (healing after 72 h) (Online Resource 4a). This evidence concerns the gene ACP5 and rickets.