In addition, 12 candidate genes (ADHFE1, AKR1B1, CHST10, ELOVL4, EYA4, FLI1, QKI, STK33, SOX5, UNC5C, and ZNF304) and eight CIMP markers (ADAMTS1, CHFR, CRABP1, NEUROG1, RUNX3, SFRP1, THBD, and TAC1) were significantly demethylated by vincristine treatment in two more CRC cell lines (Figure 3B). Here, AKR1B1 is linked to colorectal carcinoma.