The Trx1 level in lung (LC), colorectal (CRC), and kidney (KC) were detected at 37.71 ± 8.254, 32.92 ± 7.864, and 31.12 ± 7.541 ng/mL, with about a 13.5%, 17.9%, and 10.7% increase, respectively, compared to the female/male normal control (NFM). The gene discussed is TXN; the disease is laryngotracheoesophageal cleft.