As the results, we found that the expression levels of GRB2 protein in HCC tissues with the higher tumor stage (T3~4) and the positive serum AFP level were significantly lower than those with the lower tumor stage (T1~2, P=0.01, Table 1) and the negative serum AFP level (P=0.006, Table 1), respectively. This evidence concerns the gene AFP and hepatocellular carcinoma.