We detected the serum testosterone levels in three male HBV carriers groups, including 251 male AsCs (average age 43.1±7.6 years, average ALT 29.6±10.5 IU/L, average TBil 14.9±10.3 μmol/L), 48 patients with mild to moderate hepatitis B (average age 34.4±9.5 years, average ALT 659±509 IU/L, average TBil 25.0±15.4 μmol/L), and 32 patients with ALFs (average age 42.2±12.8 years, average ALT 362±538 IU/L, average TBil 358.7±229.2 μmol/L, average INR 2.39±1.32). The gene discussed is GPT; the disease is hepatitis B virus infection.