However, in the LEA and HEA group, c-kit/Ki67 double labeled cells in ICC-IM were characterized by closely adjacent cell bodies and relatively bipolar processes and the mean density reached to 18.37±1.60 mm−2 and 15.27±1.81 mm−2 (both P<0.001). This evidence concerns the gene MKI67 and intrahepatic cholangiocarcinoma.