Univariate analysis found an association of tumor-free survival with tumor size, serum AFP ≥ 400 ng/mL, BCLC, TNM stage, invasion and metastasis, and loss of 8p12-p23.2 and 19p13.1-p13.3 (all P-values ≤ 0.025) (Table 3). The gene discussed is AFP; the disease is neoplasm.