3T3-L1 adipocytes transduced with pXLG3.HA.GLUT4.GFP were examined in order to determine firstly, whether infection with lentivirus and subsequent generation of a pseudo stable cell line, altered the localisation of GLUT4 and the expression of key proteins involved in GLUT4 trafficking and secondly, whether these cells showed similar trafficking properties to electroporated cells. This evidence concerns the gene SLC2A4 and infection.