The following major findings were obtained from our study: 1) A right A-TP rabbit model induced by a pacemaker for 1-week increased the expression of miR-1; 2) A-TP induced a shortening of AERP (in vivo and in atrial cells) and a significantly increased IKs; 3) The overexpression of miR-1 via in vivo infection of recombinant LV-miRNA-1 accelerated the shortening of AERP and the increasing of IKs, and this effect was reversed by AMO-1; 4) KCNE1 and KCNB2 as the target genes for miR-1 were confirmed by the luciferase activity assay. This evidence concerns the gene KCNE1 and infection.