The co-localization of BTF3, HINT1 and NDRG1 was next investigated in the multi-labeled immunofluorescence tissue cores from relapsed and non-relapsed patients from composite fluorescent images (Figure 5; BTF3, HINT1 and NDRG1, green, blue and red, respectively) in order determine the utility of this approach for prostate cancer stratification. Here, HINT1 is linked to prostate cancer.