To examine whether the specific fibulin-5 siRNAs could suppress endogenous fibulin-5 expression in NPC cell lines, siRNAs were transfected into NPC cell lines for 24 hour, followed by Q-RT-PCR and western blot analysis using fibulin-5 Taq-Man probe and antibodies against fibulin-5. The gene discussed is FBLN5; the disease is nasopharyngeal carcinoma.